Diagnosis Archives - Microbiology Notes

Basic Microbiology, Biology, Diagnosis, Molecular Biology, Virology
HAEMAGGLUTINATION ASSAY – VIRAL QUANTITATION 4.59/5 (80)

By Sudip Dhakal on June 3, 2017

Please rate this Please Rate 0 1 2 3 4 5 HAEMAGGLUTINATION ASSAY – VIRAL QUANTITATION Haemagglutination assay was developed by American virologist George Hirst in 1941-1942. The ability of certain viruses to bind with the red blood cells through their superficial glycoproteins and proteins had been utilised to quantitate these viruses and the assay is termed as haemagglutination assay.… Continue reading "HAEMAGGLUTINATION ASSAY – VIRAL QUANTITATION <span class="rating-result after_title mr-filter rating-result-488" > <span class="mr-star-rating"> <i class="fa fa-star mr-star-full"></i> <i class="fa fa-star mr-star-full"></i> <i class="fa fa-star mr-star-full"></i> <i class="fa fa-star mr-star-full"></i> <i class="fa fa-star-half-o mr-star-half"></i> </span> <span class="star-result"> 4.59/5</span> <span class="count"> (80) </span> </span>"
Bacteriology, Biochemical Test, Diagnosis, Laboratory
Bacitracin Test- Principle, Procedure, Result with Limitation 4.61/5 (31)

By Pratiksha Pokhrel on September 30, 2015

Please rate this Please Rate 0 1 2 3 4 5 Bacitracin Test- Principle, Procedure, Result with Limitation Bacitracin is produced by Bacillus licheniformis and it interrupt the formation of peptidoglycan, a major component of bacterial cell wall. If bacitracin is present it blocks bactoprenol from transporting NAM and NAG sugars across the cell membrane thereby inhibiting further synthesis of… Continue reading "Bacitracin Test- Principle, Procedure, Result with Limitation <span class="rating-result after_title mr-filter rating-result-228" > <span class="mr-star-rating"> <i class="fa fa-star mr-star-full"></i> <i class="fa fa-star mr-star-full"></i> <i class="fa fa-star mr-star-full"></i> <i class="fa fa-star mr-star-full"></i> <i class="fa fa-star-half-o mr-star-half"></i> </span> <span class="star-result"> 4.61/5</span> <span class="count"> (31) </span> </span>"
Bacteriology, Biochemical Test, Diagnosis, Laboratory
CAMP Test- Principle, Purpose, Procedure, Result and Limitation 4.68/5 (186)

By Pratiksha Pokhrel on September 22, 2015

Please rate this Please Rate 0 1 2 3 4 5 CAMP Test- Principle, Purpose, Procedure, Result and Limitation CAMP test was first identified by Christie, Atkins, and Munch-Peterson in 1944 and CAMP test is an acronym of three researchers. CAMP (Christie, Atkins, and Munch-Peterson) test is used for the presumptive identification of Group B Streptococci (Streptococcus agalactiae). It is… Continue reading "CAMP Test- Principle, Purpose, Procedure, Result and Limitation <span class="rating-result after_title mr-filter rating-result-209" > <span class="mr-star-rating"> <i class="fa fa-star mr-star-full"></i> <i class="fa fa-star mr-star-full"></i> <i class="fa fa-star mr-star-full"></i> <i class="fa fa-star mr-star-full"></i> <i class="fa fa-star-half-o mr-star-half"></i> </span> <span class="star-result"> 4.68/5</span> <span class="count"> (186) </span> </span>"
Bacteriology, Biochemical Test, Diagnosis, Laboratory
Bile Esculin Test- Principle, Procedure, Result Interpretation and Limitation 4.64/5 (77)

By Pratiksha Pokhrel on September 1, 2015

Please rate this Please Rate 0 1 2 3 4 5 Bile Esculin Test- Principle, Procedure, Result Interpretation and Limitation Bile Esculin agar (BEA) is used for this test. BEA is a selective and differential medium which is presumptively used to identify Enterococci and group D Streptococci based on the ability of an organism to hydrolyze esculin. Bile esculin agar… Continue reading "Bile Esculin Test- Principle, Procedure, Result Interpretation and Limitation <span class="rating-result after_title mr-filter rating-result-143" > <span class="mr-star-rating"> <i class="fa fa-star mr-star-full"></i> <i class="fa fa-star mr-star-full"></i> <i class="fa fa-star mr-star-full"></i> <i class="fa fa-star mr-star-full"></i> <i class="fa fa-star-half-o mr-star-half"></i> </span> <span class="star-result"> 4.64/5</span> <span class="count"> (77) </span> </span>"
Bacteriology, Biochemical Test, Diagnosis, Laboratory
Deoxyribonuclease (DNase) Test- Principle, Uses, Procedure, Result Interpretation, Quality Control, Examples and Limitations 4.9/5 (48)

By Pratiksha Pokhrel on July 19, 2015

Please rate this Please Rate 0 1 2 3 4 5 Deoxyribonuclease (DNase) Test- Principle, Uses, Procedure, Result Interpretation, Quality Control, Examples and Limitations This test is presumptively used to differentiate Staphylococcus aureus which produces the enzyme deoxyribonuclease from other Staphylococci which do not produce deoxyribonuclease (DNase). Staphylococcus aureus possesses a heat-stable enzyme, a thermonuclease. To detect this enzyme, first… Continue reading "Deoxyribonuclease (DNase) Test- Principle, Uses, Procedure, Result Interpretation, Quality Control, Examples and Limitations <span class="rating-result after_title mr-filter rating-result-105" > <span class="mr-star-rating"> <i class="fa fa-star mr-star-full"></i> <i class="fa fa-star mr-star-full"></i> <i class="fa fa-star mr-star-full"></i> <i class="fa fa-star mr-star-full"></i> <i class="fa fa-star mr-star-full"></i> </span> <span class="star-result"> 4.9/5</span> <span class="count"> (48) </span> </span>"
Bacteriology, Biochemical Test, Diagnosis, Laboratory
PYR ( L-Pyrrolidonyl-β-Naphthylamide) Test- Principle, Uses, Procedure, Result Interpretaion, Examples and Limitation 4.92/5 (79)

By Pratiksha Pokhrel on July 7, 2015

Please rate this Please Rate 0 1 2 3 4 5 PYR ( L-Pyrrolidonyl-β-Naphthylamide) Test- Principle, Uses, Procedure, Result Interpretaion, Examples and Limitation PYR test is a rapid test which is used in the identification of group A beta-hemolytic Streptococci and in the differentiation of Enterococcus from group D Streptococci. Principle PYR test determines the activity of enzyme L-pyrroglutamyl amino-peptidase… Continue reading "PYR ( L-Pyrrolidonyl-β-Naphthylamide) Test- Principle, Uses, Procedure, Result Interpretaion, Examples and Limitation <span class="rating-result after_title mr-filter rating-result-98" > <span class="mr-star-rating"> <i class="fa fa-star mr-star-full"></i> <i class="fa fa-star mr-star-full"></i> <i class="fa fa-star mr-star-full"></i> <i class="fa fa-star mr-star-full"></i> <i class="fa fa-star mr-star-full"></i> </span> <span class="star-result"> 4.92/5</span> <span class="count"> (79) </span> </span>"
Antimicrobial Susceptibility Testing, Bacteriology, Diagnosis
E (Epsilometer) Test- Principle, Purpose, Procedure, Result Interpretation with Precaution, Advantages and Disadvantages 4.64/5 (80)

By Pratiksha Pokhrel on July 1, 2015

Please rate this Please Rate 0 1 2 3 4 5 E (Epsilometer) Test- Principle, Purpose, Procedure, Result Interpretation with Precaution, Advantages and Disadvantages The E-test has been developed to provide a direct quantification of antimicrobial susceptibility of microorganisms. E-test is a laboratory test used to determine minimum inhibitory concentration (MIC) and whether or not a specific strain of bacterium… Continue reading "E (Epsilometer) Test- Principle, Purpose, Procedure, Result Interpretation with Precaution, Advantages and Disadvantages <span class="rating-result after_title mr-filter rating-result-61" > <span class="mr-star-rating"> <i class="fa fa-star mr-star-full"></i> <i class="fa fa-star mr-star-full"></i> <i class="fa fa-star mr-star-full"></i> <i class="fa fa-star mr-star-full"></i> <i class="fa fa-star-half-o mr-star-half"></i> </span> <span class="star-result"> 4.64/5</span> <span class="count"> (80) </span> </span>"
Diagnosis, Protocol, Virology
Japanese Encephalitis (JE) ELISA Protocol 3.8/5 (5)

By Pratiksha Pokhrel on June 18, 2015

Please rate this Please Rate 0 1 2 3 4 5 Japanese Encephalitis (JE) ELISA Protocol Take the required number of micro wells from the foil pouch. Number the test strips as 1, 2, 3, 4….) Select the samples to be tested. Dilute serum sample 1:100 or CSF 1:10 in tubes using sample diluents for JE IgM. Prepare wash buffer.… Continue reading "Japanese Encephalitis (JE) ELISA Protocol <span class="rating-result after_title mr-filter rating-result-41" > <span class="mr-star-rating"> <i class="fa fa-star mr-star-full"></i> <i class="fa fa-star mr-star-full"></i> <i class="fa fa-star mr-star-full"></i> <i class="fa fa-star mr-star-full"></i> <i class="fa fa-star-o mr-star-empty"></i> </span> <span class="star-result"> 3.8/5</span> <span class="count"> (5) </span> </span>"
Diagnosis, Protocol, Virology
Diagnosis of Dengue by IgM capture ELISA with principle, procedure and result interpretation 4.38/5 (16)

By Pratiksha Pokhrel on June 11, 2015

Please rate this Please Rate 0 1 2 3 4 5 Diagnosis of Dengue by IgM capture ELISA with principle, procedure and result interpretation Description of the test Dengue IgM Capture ELISA is an enzyme linked immunosorbent assay for the qualitative detection of IgM antibodies against dengue virus antigen in human serum. Dengue IgM Capture ELISA is for the qualitative… Continue reading "Diagnosis of Dengue by IgM capture ELISA with principle, procedure and result interpretation <span class="rating-result after_title mr-filter rating-result-18" > <span class="mr-star-rating"> <i class="fa fa-star mr-star-full"></i> <i class="fa fa-star mr-star-full"></i> <i class="fa fa-star mr-star-full"></i> <i class="fa fa-star mr-star-full"></i> <i class="fa fa-star-half-o mr-star-half"></i> </span> <span class="star-result"> 4.38/5</span> <span class="count"> (16) </span> </span>"